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Choudhury, Prasanta Kumar
- Cardioprotective Activity of Ocimum canum Hydro-Alcoholic Leaf Extracts against Isoproterenol Induced Myocardial Infarction in Rats
Abstract Views :275 |
PDF Views:2
Authors
Saiprasanna Behera
1,
S. Manohar Babu
2,
Y. Roja Ramani
3,
Prasanta Kumar Choudhury
1,
Rajeshree Panigrahi
1
Affiliations
1 Department of Pharmacology, Royal College of Pharmacy and Health Sciences, Brahmapur, Odisha- 760002, IN
2 Department of Pharmacology, SIMS College of Pharmacy, Mangaldas Nagar, Guntur- 522001, IN
3 Department of Pharmacology, MKCG Medical College, Berhampur, Odisha- 760004, IN
1 Department of Pharmacology, Royal College of Pharmacy and Health Sciences, Brahmapur, Odisha- 760002, IN
2 Department of Pharmacology, SIMS College of Pharmacy, Mangaldas Nagar, Guntur- 522001, IN
3 Department of Pharmacology, MKCG Medical College, Berhampur, Odisha- 760004, IN
Source
Research Journal of Pharmacology and Pharmacodynamics, Vol 4, No 4 (2012), Pagination: 191-201Abstract
Myocardial infarction (MI) was produced in rats with 200 mg/kg of isoproterenol (ISO) administered subcutaneously (sc) twice at an interval of 24 h. Shift in antioxidant parameters, lactate dehydrogenase (LDH), aspartate aminotransferase (AST), alanine aminotransferase (ALT), creatine phosphokinase (CPK), Troponin T, Catalase together with morphological and histopathological changes were investigated. Two hundred mg/kg ISO dose was selected for the present study as this dose offered significant alteration in biochemical parameters along with moderate necrosis in heart. Effect of pre- treatment of hydro-alcoholic extract of Ocimum canum (OC) at different doses (100, 200 and 400 mg/kg) was investigated against ISO (200 mg/kg) induced myocardial infarction in rats. Modulation of various biochemical parameters and membrane integrity was studied. OC at the dose of 200 and 400 mg/kg reduced significantly glutathione (GSH), superoxide dismutase (SOD) and LDH levels. It also inhibited the lipid peroxidation as observed by the reduced thiobarbituric acid reactive substances (TBARS) levels. In the present study OC at the dose of 400 mg/kg was found to demonstrate maximum cardio-protective effect. Above results were further confirmed by histopathological findings. Thus from the present study it is concluded that OC may be of therapeutic and prophylactic value in the treatment of MI.Keywords
Isoproterenol, Myocardial Infarction, Ocimum canum, Antioxidant Enzymes.
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- Formulation and In-Vitro Evaluation of Ethyl Cellulose Microspheres by Novel Quasiemulsification Method for Colonic Delivery of Metronidazole
Abstract Views :189 |
PDF Views:0
Authors
Prasanta Kumar Choudhury
1,
Padala Narasimha Murthy
2,
Suvakanta Dash
3,
Rana Mazumder
4,
Dhruba Sankar Goswami
5
Affiliations
1 Department of Pharmaceutical Technology, Royal College of Pharmacy and Health Sciences, Andhapasara Road, Berhampur-760002, Ganjam, Orissa, IN
2 Department of Pharmaceutical Technology, Royal College of Pharmacy and Health Sciences, Andhapasara Road, Berhampur-760002, Ganjam, Orissa,, IN
3 Girjananda Choudhury Institute of Pharmaceutical Sciences, Guwahati, Assam, IN
4 Dept. of Pharmaceutics, Calcutta Institute of Pharmaceutical Technology and Allied Health Sciences, Howrah-711316, West Bengal, IN
5 Dept. of Pharmaceutics, S.D. College of Pharmacy, Barnala, Punjab, IN
1 Department of Pharmaceutical Technology, Royal College of Pharmacy and Health Sciences, Andhapasara Road, Berhampur-760002, Ganjam, Orissa, IN
2 Department of Pharmaceutical Technology, Royal College of Pharmacy and Health Sciences, Andhapasara Road, Berhampur-760002, Ganjam, Orissa,, IN
3 Girjananda Choudhury Institute of Pharmaceutical Sciences, Guwahati, Assam, IN
4 Dept. of Pharmaceutics, Calcutta Institute of Pharmaceutical Technology and Allied Health Sciences, Howrah-711316, West Bengal, IN
5 Dept. of Pharmaceutics, S.D. College of Pharmacy, Barnala, Punjab, IN
Source
Research Journal of Pharmaceutical Dosage Form and Technology, Vol 2, No 4 (2010), Pagination: 295-300Abstract
The present study was aimed to develop and evaluate different polymeric microspheres for colon-specific delivery of Metronidazole for better treatment of colonic diseases. Different approaches for colon-specific drug delivery have been studied over the last decade, including pro-drugs, polymeric coating using pH sensitive or bacterial degradable polymers and matrices. In this research work we present colon-specific ethyl cellulose microspheres to deliver active molecules to the colonic region, which combine pH-dependent and controlled release properties. Microspheres were prepared by modified Novel Quasiemulsification solvent-diffusion method to study the effect of ethyl cellulose on drug release with different proportions of metronidazole and ethyl cellulose. Prepared microspheres of ethyl cellulose were evaluated for size, morphology, sphericity study, percentage yield, loose surface crystal study, drug content and entrapment efficiency. In vitro drug release study was conducted by buffer change method to mimic Gastro Intestinal environment. The investigations revealed that microspheres prepared with metronidazole: ethyl cellulose ratio (1:2) show only 19.394 ±0.67% drug release in first 5 hours and 46.72 ±0.69% in 12 hours, which prove the potentiality of ethyl cellulose for colonic delivery of drugs.Keywords
Colon Drug Delivery, Controlled-release, Ph-dependent Release, Novel Quasiemulsification Solvent-Diffusion Technique.- A Comparative Study on Phytochemical Investigation and Antioxidant Activity of Poly Herbal Mixture of Ocimum canum and Pongamia pinnata Hydro-Alcoholic Leaf Extracts
Abstract Views :204 |
PDF Views:0
Authors
Affiliations
1 Department of Pharmacology, Royal College of Pharmacy and Health Sciences, Berhmapur, Odisha- 760002, IN
2 Department of Pharmacology, SIMS College of Pharmacy, Mangaldas Nagar, Guntur- 522001, IN
3 Department of Pharmacology, MKCG Medical College, Berhampur, Odisha- 760004, IN
1 Department of Pharmacology, Royal College of Pharmacy and Health Sciences, Berhmapur, Odisha- 760002, IN
2 Department of Pharmacology, SIMS College of Pharmacy, Mangaldas Nagar, Guntur- 522001, IN
3 Department of Pharmacology, MKCG Medical College, Berhampur, Odisha- 760004, IN
Source
Research Journal of Pharmacognosy and Phytochemistry, Vol 4, No 5 (2012), Pagination: 250-261Abstract
Free radicals are implicated for many diseases including diabetes mellitus, arthritis, cancer, aging, etc. In the treatment of these diseases, antioxidant therapy has gained utmost importance. Reactive oxygen Species (ROS) is a metabolic side product of oxidative stress process which causes several diseases like atherosclerosis, cancer, etc. In defense of ROS, anti-oxidants play a key role in combatting them. As the process of aging increases the level of anti-oxidant in our body decreases and thereby needs utmost attention for its repair process which is generally administered externally. Many herbal remedies individually or in combination have been recommended in various medical expositions for the cure of different diseases. Plant products serve a best source for controlling these activities by its own metabolic pathway. Currently there has been an increased interest globally to identify antioxidant compound that are pharmacologically potent and have low or no side effects. As plants are source of natural antioxidants, much attention has been gain to plants. The quest for natural antioxidants for dietary, cosmetic and pharmaceutical uses has become a major industrial and scientific research challenges over the last two decades. A variety of free radical scavenging antioxidants exists within the body in which many of them are derived from dietary sources like fruits, vegetables and teas. Thus in this aspect three plants namely Pongamia pinnata (Karanj) and Ocimum canum (KalaTulsi) were taken up for the study. Both the plants have been recognized in different system of traditional medicines for the treatment of different diseases and ailments of human beings. In this study, Antioxidant activity of all the hydro-alcoholic extracts was determined by various antioxidant assays. In all the testing, a significant correlation existed between concentrations of the extract and percentage inhibition of free radicals. These findings suggest that the hydro-alcoholic extracts are able to scavenge free radicals, by either hydrogen or electron donating mechanisms, and can therefore act as primary antioxidants. The antioxidant property may be related to the phenols and flavonoids present in the extracts. When all three extracts were mixed together in equal proportion, in same concentration it gives synergistic effect in percentage inhibition with respect to antioxidant property.